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scaav sgrna gfp  (Addgene inc)


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    Structured Review

    Addgene inc scaav sgrna gfp
    Scaav Sgrna Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sgrna+plasmid/scAAV+GFP+(Plasmid+%2321893)/pmc13022658-113-6-7
    Average 92 stars, based on 7 article reviews
    scaav sgrna gfp - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: CTCF-RNA interactions orchestrate cell-specific chromatin loop organization
    Article Snippet: The WT and ΔZF1 donor plasmids were generated by modifying an existing plasmid (Addgene 156432) that contains a constitutively expressed rtta3G cassette and a dox-inducible CTCF, flanked by Tigre homology arms (see Table S9 for details). .. The donor plasmid and sgRNA plasmid targeting the Tigre locus (Addgene 92144) were transfected into the C7-E10 clone. ..

    Article Title: Short activation domains control chromatin association of transcription factors
    Article Snippet: .. Each well received 1 μg of a combined Cas9 and sgRNA plasmid targeting the AAVS1 locus (Addgene plasmid #227272) and 2 μg of repair template containing the reporter locus, similar to that of the previously described construct ( ). .. The reporter construct is based on a published reporter (Addgene #176294), but we replaced puromycin resistance with blasticidin resistance, added VKYY to the start of the GFP to boost translation , inserted a small t intron, and inserted a WPRE before the SV40 poly(A) signal.

    Article Title: Tuft cells act as regenerative stem cells in the human intestine.
    Article Snippet: Ligations were transformed into MACH1T1 cells (Thermo Fisher) and sgRNA identity was confirmed by Sanger sequencing. .. For electroporation, 2.5 μg sgRNA plasmid (BPK1520, Addgene no. 65777), 7.5 μg pCMV_ AncBE4max_P2A_GFP plasmid (for base-editing, Addgene no. 112100) or pCAS9-mCherry-Frame +1 plasmid (for conventional CRISPR–Cas9, Addgene no. 66940), together with 10 μg PiggyBac transposon system (5 μg transposase + 5 μg hygromycin resistance containing transposon)43 were co-electroporated into human duodenum, ileum and colon AVIL-Clover reporter organoids. ..

    Article Title: CTCF-RNA interactions orchestrate cell-specific chromatin loop organization
    Article Snippet: A plasmid containing WT OsTIR1 flanked by Rosa26 homology arms (Addgene 86233) was modified to express OsTIR1(F74G) (see Table S9 for details). .. Clone C7 was transfected with the OsTIR1(F74G) donor plasmid and sgRNA plasmid targeting the Rosa26 locus (Addgene 64216). ..

    Article Title: CTCF-RNA interactions orchestrate cell-specific chromatin loop organization
    Article Snippet: A plasmid expressing WT OsTIR1 flanked by Rosa26 homology arms (Addgene, 86233) was modified to express OsTIR1(F74G) instead (see table S9 for details). .. Clone C7 was transfected with the KL063–pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26 donor plasmid (Addgene, 232929) and sgRNA plasmid targeting the Rosa26 locus (Addgene, 64216). ..

    Article Title: Tuft cells act as regenerative stem cells in the human intestine
    Article Snippet: Ligations were transformed into MACH1T1 cells (Thermo Fisher) and sgRNA identity was confirmed by Sanger sequencing. .. For electroporation, 2.5 μg sgRNA plasmid (BPK1520, Addgene no. 65777), 7.5 μg pCMV_AncBE4max_P2A_GFP plasmid (for base-editing, Addgene no. 112100) or pCAS9-mCherry-Frame +1 plasmid (for conventional CRISPR–Cas9, Addgene no. 66940), together with 10 μg PiggyBac transposon system (5 μg transposase + 5 μg hygromycin resistance containing transposon) were co-electroporated into human duodenum, ileum and colon AVIL-Clover reporter organoids. ..

    Article Title: Generation of a Brachyury reporter cell line (BJNhem20 Brachyury (TBXT)-2A-EGFP) in human embryonic stem cells using CRISPR-Cas9 gene targeting
    Article Snippet: .. Transfection was carried out using Lipofectamine Stem Transfection Reagent (Thermo Fisher Scientific, STEM0001), with a 1:2 molar ratio of sgRNA plasmid to donor plasmid (Addgene #83344; Chu et al., 2016). ..

    Article Title: Compositions and methods for making epigenetic modifications
    Article Snippet: A double-cut HDR donor plasmid with the mScarlet tag sequence flanked by 800 bp homology arms was cloned from a pUC19 backgone (Addgene #50005) using NEBuilder HiFi DNA Assembly (New England BioLabs, E2621L). .. The sgRNA sequence targeting CLTA was ligated into pX458 (Addgene #48138) to generate the Cas9+sgRNA plasmid. .. The HDR donor and Cas9+sgRNA plasmids were co-transfected into HEK293T cells using TransIT-LT1 Transfection Reagent (Mirus Bio, 10767-122). mScarlet+ cells were sorted by FACS 6 days post transfection and successful tag insertion was validated via PCR.

    Transfection:

    Article Title: CTCF-RNA interactions orchestrate cell-specific chromatin loop organization
    Article Snippet: The WT and ΔZF1 donor plasmids were generated by modifying an existing plasmid (Addgene 156432) that contains a constitutively expressed rtta3G cassette and a dox-inducible CTCF, flanked by Tigre homology arms (see Table S9 for details). .. The donor plasmid and sgRNA plasmid targeting the Tigre locus (Addgene 92144) were transfected into the C7-E10 clone. ..

    Article Title: CTCF-RNA interactions orchestrate cell-specific chromatin loop organization
    Article Snippet: A plasmid containing WT OsTIR1 flanked by Rosa26 homology arms (Addgene 86233) was modified to express OsTIR1(F74G) (see Table S9 for details). .. Clone C7 was transfected with the OsTIR1(F74G) donor plasmid and sgRNA plasmid targeting the Rosa26 locus (Addgene 64216). ..

    Article Title: CTCF-RNA interactions orchestrate cell-specific chromatin loop organization
    Article Snippet: A plasmid expressing WT OsTIR1 flanked by Rosa26 homology arms (Addgene, 86233) was modified to express OsTIR1(F74G) instead (see table S9 for details). .. Clone C7 was transfected with the KL063–pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26 donor plasmid (Addgene, 232929) and sgRNA plasmid targeting the Rosa26 locus (Addgene, 64216). ..

    Article Title: Generation of a Brachyury reporter cell line (BJNhem20 Brachyury (TBXT)-2A-EGFP) in human embryonic stem cells using CRISPR-Cas9 gene targeting
    Article Snippet: .. Transfection was carried out using Lipofectamine Stem Transfection Reagent (Thermo Fisher Scientific, STEM0001), with a 1:2 molar ratio of sgRNA plasmid to donor plasmid (Addgene #83344; Chu et al., 2016). ..

    Construct:

    Article Title: Short activation domains control chromatin association of transcription factors
    Article Snippet: .. Each well received 1 μg of a combined Cas9 and sgRNA plasmid targeting the AAVS1 locus (Addgene plasmid #227272) and 2 μg of repair template containing the reporter locus, similar to that of the previously described construct ( ). .. The reporter construct is based on a published reporter (Addgene #176294), but we replaced puromycin resistance with blasticidin resistance, added VKYY to the start of the GFP to boost translation , inserted a small t intron, and inserted a WPRE before the SV40 poly(A) signal.

    Electroporation:

    Article Title: Tuft cells act as regenerative stem cells in the human intestine.
    Article Snippet: Ligations were transformed into MACH1T1 cells (Thermo Fisher) and sgRNA identity was confirmed by Sanger sequencing. .. For electroporation, 2.5 μg sgRNA plasmid (BPK1520, Addgene no. 65777), 7.5 μg pCMV_ AncBE4max_P2A_GFP plasmid (for base-editing, Addgene no. 112100) or pCAS9-mCherry-Frame +1 plasmid (for conventional CRISPR–Cas9, Addgene no. 66940), together with 10 μg PiggyBac transposon system (5 μg transposase + 5 μg hygromycin resistance containing transposon)43 were co-electroporated into human duodenum, ileum and colon AVIL-Clover reporter organoids. ..

    Article Title: Tuft cells act as regenerative stem cells in the human intestine
    Article Snippet: Ligations were transformed into MACH1T1 cells (Thermo Fisher) and sgRNA identity was confirmed by Sanger sequencing. .. For electroporation, 2.5 μg sgRNA plasmid (BPK1520, Addgene no. 65777), 7.5 μg pCMV_AncBE4max_P2A_GFP plasmid (for base-editing, Addgene no. 112100) or pCAS9-mCherry-Frame +1 plasmid (for conventional CRISPR–Cas9, Addgene no. 66940), together with 10 μg PiggyBac transposon system (5 μg transposase + 5 μg hygromycin resistance containing transposon) were co-electroporated into human duodenum, ileum and colon AVIL-Clover reporter organoids. ..

    CRISPR:

    Article Title: Tuft cells act as regenerative stem cells in the human intestine.
    Article Snippet: Ligations were transformed into MACH1T1 cells (Thermo Fisher) and sgRNA identity was confirmed by Sanger sequencing. .. For electroporation, 2.5 μg sgRNA plasmid (BPK1520, Addgene no. 65777), 7.5 μg pCMV_ AncBE4max_P2A_GFP plasmid (for base-editing, Addgene no. 112100) or pCAS9-mCherry-Frame +1 plasmid (for conventional CRISPR–Cas9, Addgene no. 66940), together with 10 μg PiggyBac transposon system (5 μg transposase + 5 μg hygromycin resistance containing transposon)43 were co-electroporated into human duodenum, ileum and colon AVIL-Clover reporter organoids. ..

    Article Title: Tuft cells act as regenerative stem cells in the human intestine
    Article Snippet: Ligations were transformed into MACH1T1 cells (Thermo Fisher) and sgRNA identity was confirmed by Sanger sequencing. .. For electroporation, 2.5 μg sgRNA plasmid (BPK1520, Addgene no. 65777), 7.5 μg pCMV_AncBE4max_P2A_GFP plasmid (for base-editing, Addgene no. 112100) or pCAS9-mCherry-Frame +1 plasmid (for conventional CRISPR–Cas9, Addgene no. 66940), together with 10 μg PiggyBac transposon system (5 μg transposase + 5 μg hygromycin resistance containing transposon) were co-electroporated into human duodenum, ileum and colon AVIL-Clover reporter organoids. ..

    Sequencing:

    Article Title: Compositions and methods for making epigenetic modifications
    Article Snippet: A double-cut HDR donor plasmid with the mScarlet tag sequence flanked by 800 bp homology arms was cloned from a pUC19 backgone (Addgene #50005) using NEBuilder HiFi DNA Assembly (New England BioLabs, E2621L). .. The sgRNA sequence targeting CLTA was ligated into pX458 (Addgene #48138) to generate the Cas9+sgRNA plasmid. .. The HDR donor and Cas9+sgRNA plasmids were co-transfected into HEK293T cells using TransIT-LT1 Transfection Reagent (Mirus Bio, 10767-122). mScarlet+ cells were sorted by FACS 6 days post transfection and successful tag insertion was validated via PCR.



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